Shrikant L. Patil1*,
Tanhaji Ghodke2, Shilpa M. Patil3, Swaroop K4,
H. M. Somashekarappa5
1Assistant Professor, Department
of Physiology, K. S. Hegde Medical Academy, Nitte University, Mangalore, India
2Junior Research Fellow, Department of Biophysics, Govt.
Institute of Science, Aurangabad, India
3Assistant Professor, Department of Biophysics,
University of Mumbai, Kalina Campus, Mumbai India
4Centre for Application of Radioisotopes and
Radiation Technology, USIC, Mangalore University, Mangalore, India
5Centre for Application of Radioisotopes and
Radiation Technology, USIC, Mangalore University, Mangalore, India
*Address for Correspondence: Dr.
Shrikant L. Patil, Asst. Professor, Department of Physiology, K. S.
Hegde Medical Academy, Nitte University, Mangalore, India
ABSTRACT- Radioprotective mechanisms of Rutin (RUT) and Quercetin
(QRT) against gamma radiation was studied by
investigating recovery of histopathology of intestinal mucosa and bone marrow
in Swiss albino mice. These mice were treated with RUT (10mg/kg.b.wt.) and QRT (20mg/kg.b.wt.) once daily for five consecutive
days and exposed to 7.5 Gy of gamma radiation after the last administration. RUT
and QRT
treatment before exposure to 7.5 Gy of gamma radiation. To assess the
intestinal and bone marrow protective potential of RUT and QRT, histological
analysis was carried out by observing the villus height, crypt survival, number
of goblet cells/villus section and dead cells/villus section in the mouse
jejunum and bone marrow cellularity at 24 hours post-irradiation. Mice exposed
gamma radiation caused a significant decline in the villus height and crypt
number with an increase in goblet and dead cell number with a significant
decrease in bone marrow nucleated cells. The potent antioxidant nature of RUT
and QRT mitigate the oxidative stress induced by gamma radiation and thus
protect the mice from gastrointestinal damage.
Key-words- Rutin,
Quercetin, Cytoprotective, Irradiation
