Santanu Maiti1, Pranamita Biswas2,
Sukanya Banerjee2, Nandan Kumar
Jana3*
1B.Tech, Department
of Biotechnology, Heritage Institute of Technology, Kolkata, West Bengal, India
2M.Tech, Department of Biotechnology, Heritage
Institute of Technology, Kolkata, West Bengal, India
3Assistant Professor, Department of
Biotechnology, Heritage Institute of Technology, Kolkata, West Bengal, India
*Address for Correspondence: Dr. Nandan
Kumar Jana, Assistant Professor, Department of Biotechnology, Heritage
Institute of Technology, 994, Chowbaga Road, Anandapur, Kolkata-700107, West
Bengal, India
ABSTRACT- Coronary artery disease (CAD) is
suspected as a leading cause of mortality in developed countries. Due to
cholesterol and fat deposit plaque is forming into the inner walls of the
arteries of the heart, which leads to narrowing of blood vessels of heart and
reduce the blood flow rate into heart. Proprotein convertase subtilisin-like
kexin type 9 (PCSK9) is one of the candidate gene that regulate lipoprotein
retention pathway of CAD development. It is a newly discovered serine protease
that plays a key role in LDL-C homeostasis by mediating LDL receptor
(LDLR). The LDL receptor is breakdown
through a post transcriptional mechanism and induces the production of very
low-density lipoprotein in the fasting state. The aim of this study was to
investigate the frequency of single nucleotide polymorphism (SNP) of PCSK9 gene
of 155 CAD patients and 102 ages matched healthy controls. Serum lipids
including total cholesterol (TC), triglycerides (TG), HDL, LDL, and VLDL were
analyzed. PCR-RFLP analysis was carried out to genotype regions carrying Eam 1104I restriction site in the PCSK9.
Gene considering significant difference in serum TC, TG, HDL-C, LDL-C and
VLDL-C levels (P<0.001, <0.0001) of patients and control samples. In CAD
patients, G allele frequency is less than A allele frequency. G allele is responsible
for decreasing the LDL: HDL ratio which shows evidence in having its protecting
effect on the occurrence of CAD in West Bengal Population.
Key-words- CAD, PCSK9, SNP, Eam1104I, Polymorphism, West Bengal population
