Tuesday, September 12, 2017

Biotransformation of Phenol to L-tyrosine with Resting Cells of Citrobacter freundii MTCC 2424

Vandna Kumari*
Assistant Professor, Department of Botany, Abhilashi PG Institute of Sciences, Nerchowk, Distt. Mandi, HP, India
*Address for Correspondence: Ms. Vandna Kumari, Asst. Professor, Department of Botany, Abhilashi PG Institute of Sciences, Nerchowk, Distt. Mandi- 175008 HP, India


ABSTRACT- In this present study, the biotransformation of phenol to L-tyrosine was studied with resting cells of Citrobacter freundii MTCC 2424 containing high tyrosine phenol lyase activity. Different process parameters leading to synthesis of L-tyrosine were optimized. The L-tyrosine formed from biotransformation reactions was detected and quantified by HPLC technique. The maximum L-tyrosine conversion 69% (6.49g/l) was obtained with ammonium chloride 0.25M, phenol 0.1M, and sodium pyruvate 0.2M in borate buffer (0.1M) of pH 8.5 at 35°C temperature for 45min of incubation. Higher phenol concentration was found to be inhibitory for biotransformation due to phenol inactivation of catalyst.

Key-words- Citrobacter freundii MTCC 2424, L-tyrosine, Tyrosine phenol lyase, Biotransformation